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Sometimes observations may be made on non-dividing (interphase) cells.

The sex of an unborn fetus can be determined by observation of interphase cells (see amniotic centesis and Barr body).

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There is variation between species in chromosome number, and in detailed organization, despite their construction from the same macromolecules.

This variation provides the basis for a range of studies in evolutionary cytology.

For humans, white blood cells are used most frequently because they are easily induced to divide and grow in tissue culture.The next stage took place after the development of genetics in the early 20th century, when it was appreciated that chromosomes (that can be observed by karyotype) were the carrier of genes.Lev Delaunay is applied after cells have been arrested during cell division by a solution of colchicine usually in metaphase or prometaphase when most condensed.Karyotypes describe the chromosome count of an organism and what these chromosomes look like under a light microscope.Attention is paid to their length, the position of the centromeres, banding pattern, any differences between the sex chromosomes, and any other physical characteristics.

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Any variation from the standard karyotype may lead to developmental abnormalities.

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